TTC7A Polyclonal antibody
TTC7A Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 21600-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, PC-3 cells, mouse small intestine tissue, mouse colon tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
21600-1-AP targets TTC7A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TTC7A fusion protein Ag16212 相同性解析による交差性が予測される生物種 |
| Full Name | tetratricopeptide repeat domain 7A |
| Calculated molecular weight | 858 aa, 96 kDa |
| Observed molecular weight | 96 kDa |
| GenBank accession number | BC114365 |
| Gene symbol | TTC7A |
| Gene ID (NCBI) | 57217 |
| RRID | AB_10733221 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TTC7A (TPR repeat protein 7A) is expressed in enterocytes within the duodenum, ileum, and colon, and has a role in enterocyte survival and function. Mutations in the TTC7A gene can result in a spectrum of intestinal disease, including multiple intestinal atresia (MIA) and very early onset inflammatory bowel diseases (VEOIBD). Functional analysis revealed that TTC7A binds to and facilitates the transport of PI4KIIIa from the trans-Golgi to the plasma membrane, while loss of TTC7A results in dysregulation of PI4KIIIa signaling. This antibody specially recognizes endogenous TTC7A. (24417819)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TTC7A antibody 21600-1-AP | Download protocol |
| IHC protocol for TTC7A antibody 21600-1-AP | Download protocol |
| IP protocol for TTC7A antibody 21600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gastroenterology Mutations in tetratricopeptide repeat domain 7A result in a severe form of very early onset inflammatory bowel disease. | ||
Front Immunol A Novel Homozygous TTC7A Missense Mutation Results in Familial Multiple Intestinal Atresia and Combined Immunodeficiency. | ||
J Clin Immunol Bialellic Mutations in Tetratricopeptide Repeat Domain 7A (TTC7A) Cause Common Variable Immunodeficiency-Like Phenotype with Enteropathy. |
