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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BBS8 Polyclonal antibody

BBS8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12505-1-AP

Synonyms

BBS8, TPR repeat protein 8, TTC8

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using BBS8 Polyclonal antibody (12505-1-AP)

    WB analysis of mouse testis using 12505-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using BBS8 Polyclonal antibody (12505-1-AP)

    WB analysis of mouse testis using 12505-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BBS8 Polyclonal antibody (12505-1-AP)

    WB analysis of NIH/3T3 using 12505-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using BBS8 Polyclonal antibody (12505-1-AP)

    WB analysis of human lung using 12505-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using BBS8 Polyclonal antibody (12505-1-AP)

    IP experiment of HeLa using 12505-1-AP

    IP result of anti-BBS8 (IP:12505-1-AP, 3ug; Detection:12505-1-AP 1:400) with HeLa cells lysate 2000ug.

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Tested Applications

Positive WB detected inmouse testis tissue, human lung tissue, NIH/3T3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12505-1-AP targets BBS8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS8 fusion protein Ag3186 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 8
Calculated molecular weight 531 aa, 60 kDa
Observed molecular weight 50-52 kDa
GenBank accession numberBC026351
Gene symbol BBS8
Gene ID (NCBI) 123016
RRIDAB_2211953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.

Protocols

Product Specific Protocols
WB protocol for BBS8 antibody 12505-1-APDownload protocol
IP protocol for BBS8 antibody 12505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols