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Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using TTF2 Polyclonal antibody (13722-1-AP)

    WB analysis of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TTF2 Polyclonal antibody (13722-1-AP)

    WB analysis using 13722-1-AP (same clone as 13722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A2780 cells using TTF2 Polyclonal antibody (13722-1-AP)

    WB analysis of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A2780 cells using TTF2 Polyclonal antibody (13722-1-AP)

    IP experiment of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    IP result of anti-TTF2 (IP:13722-1-AP, 4ug; Detection:13722-1-AP 1:500) with A2780 cells lysate 840ug. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TTF2 Polyclonal antibody (13722-1-AP)

    IHC staining of human ovary tumor using 13722-1-AP (same clone as 13722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TTF2 Polyclonal antibody (13722-1-AP)

    IHC staining of human ovary tumor using 13722-1-AP (same clone as 13722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TTF2 Polyclonal antibody (13722-1-AP)

    IF Staining of HeLa using 13722-1-AP (same clone as 13722-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (black). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TTF2 Polyclonal antibody (13722-1-AP)

    IF Staining of HeLa using 13722-1-AP (same clone as 13722-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

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TTF2 Polyclonal antibody, PBS Only

TTF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13722-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13722-1-PBS targets TTF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTF2 fusion protein Ag4671 相同性解析による交差性が予測される生物種
Full Name transcription termination factor, RNA polymerase II
Calculated molecular weight 1162 aa, 129 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC030058
Gene Symbol TTF2
Gene ID (NCBI) 8458
RRIDAB_2262865
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNY4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].