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Species Reactivity

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  • Featured Product
  • KD/KO Validated

TTK Polyclonal antibody

TTK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10381-1-AP

Synonyms

MPS1L1, MPS1, Mps 1, hMPS1, ESK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTK Polyclonal antibody (10381-1-AP)

    WB analysis using 10381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TTK Polyclonal antibody (10381-1-AP)

    WB analysis of MCF-7 using 10381-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TTK Polyclonal antibody (10381-1-AP)

    WB analysis of HEK-293 using 10381-1-AP

    WB result of TTK antibody (10381-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTK transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of DU 145 cells using TTK Polyclonal antibody (10381-1-AP)

    IP experiment of DU 145 using 10381-1-AP

    IP result of anti-TTK (IP:10381-1-AP, 4ug; Detection:10381-1-AP 1:600) with DU 145 cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human breast cancer using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10381-1-AP (TTK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of mouse colon using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of mouse colon using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human liver cancer using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human liver cancer using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human testis using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human testis using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human kidney using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human kidney using 10381-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TTK Polyclonal antibody (10381-1-AP)

    IF Staining of HeLa using 10381-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTK antibody (10381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using TTK Polyclonal antibody (10381-1-AP)

    FC experiment of HeLa using 10381-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TTK (10381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, MCF-7 cells, HeLa cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human liver cancer tissue, human testis tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10381-1-AP targets TTK in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTK fusion protein Ag0405 相同性解析による交差性が予測される生物種
Full Name TTK protein kinase
Calculated molecular weight 97 kDa
Observed molecular weight 96-97 kDa
GenBank accession numberBC000633
Gene symbol TTK
Gene ID (NCBI) 7272
RRIDAB_2211979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).

Protocols

Product Specific Protocols
WB protocol for TTK antibody 10381-1-APDownload protocol
IHC protocol for TTK antibody 10381-1-APDownload protocol
IF protocol for TTK antibody 10381-1-APDownload protocol
IP protocol for TTK antibody 10381-1-APDownload protocol
FC protocol for TTK antibody 10381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Cell Biol

PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size

Authors - Tomoaki Sobajima
View Article

Oxid Med Cell Longev

Oxidative Stress Delays Prometaphase/Metaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint.

Authors - Que Wu
View Article
humanWB,IF

J Nanobiotechnology

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Authors - Linjia Su
View Article
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
View Article
humanIHC

Front Oncol

Identification and Validation of Hub Genes Associated with Bladder Cancer by Integrated Bioinformatics and Experimental Assays

Authors - Kang Chen
View Article
humanIHC

Front Mol Biosci

Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.

Authors - Yongqu Lu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Zhiping (Verified Customer) (07-28-2022)

Very clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500