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  • Featured Product
  • KD/KO Validated

TTL Monoclonal antibody, PBS Only

TTL Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E5F8

Cat no : 66076-1-PBS

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 2E5F8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTL Monoclonal antibody (66076-1-Ig)

    WB analysis using 66076-1-Ig (same clone as 66076-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using TTL Monoclonal antibody (66076-1-Ig)

    WB analysis of fetal human brain using 66076-1-Ig (same clone as 66076-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TTL Monoclonal antibody (66076-1-Ig)

    WB analysis of SH-SY5Y using 66076-1-Ig (same clone as 66076-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TTL Monoclonal antibody (66076-1-Ig)

    IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TTL Monoclonal antibody (66076-1-Ig)

    IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66076-1-PBS targets TTL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TTL fusion protein Ag4708 相同性解析による交差性が予測される生物種
Full Name tubulin tyrosine ligase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC036819
Gene symbol TTL
Gene ID (NCBI) 150465
RRIDAB_11182924
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.