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  • Featured Product
  • KD/KO Validated

TTL Recombinant antibody

TTL Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3P9

Cat no : 82763-5-RR

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTL Recombinant antibody (82763-5-RR)

    WB analysis using 82763-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82763-5-RR (TTL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TTL Recombinant antibody (82763-5-RR)

    WB analysis of HeLa using 82763-5-RR

    WB result of TTL antibody (82763-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells.

  • Affinity and Kinetic Characterization of 82763-5-RR

    Affinity and Kinetic Characterization of 82763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82763-5-RR against Human TTL were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, U2OS cells, Y79 cells, Jurkat cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82763-5-RR targets TTL in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TTL fusion protein Ag4526 相同性解析による交差性が予測される生物種
Full Name tubulin tyrosine ligase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC036819
Gene symbol TTL
Gene ID (NCBI) 150465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Protocols

Product Specific Protocols
WB protocol for TTL antibody 82763-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols