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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTLL12 Polyclonal antibody

TTLL12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21447-1-AP

Synonyms

Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTLL12 Polyclonal antibody (21447-1-AP)

    WB analysis using 21447-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21447-1-AP (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

21447-1-AP targets TTLL12 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTLL12 fusion protein Ag13804 相同性解析による交差性が予測される生物種
Full Name tubulin tyrosine ligase-like family, member 12
Calculated molecular weight 644 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC001070
Gene symbol TTLL12
Gene ID (NCBI) 23170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).

Protocols

Product Specific Protocols
WB protocol for TTLL12 antibody 21447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Clin Exp Pathol

TTLL12 expression in ovarian cancer correlates with a poor outcome.

Authors - Shangjie Yang
View Article