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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using TTPA Monoclonal antibody (67431-1-Ig)

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using TTPA Monoclonal antibody (67431-1-Ig)

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TTPA Monoclonal antibody (67431-1-Ig)

    WB analysis of pig liver using 67431-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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TTPA Monoclonal antibody

TTPA Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67431-1-Ig
Clone No.3E7D10

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

Alpha TTP, alphaTTP, ATTP, AVED, TPP1, TTP1, TTPA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat liver tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67431-1-Ig targets TTPA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TTPA fusion protein Ag13587 相同性解析による交差性が予測される生物種
Full Name tocopherol (alpha) transfer protein
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC058000
Gene Symbol TTPA
Gene ID (NCBI) 7274
RRIDAB_2882669
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49638
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TTPA antibody 67431-1-IgDownload protocol
IHC protocol for TTPA antibody 67431-1-IgDownload protocol
IF protocol for TTPA antibody 67431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols