Prealbumin/transthyretin Polyclonal antibody

Name: Prealbumin/transthyretin Polyclonal antibody
Brand: Proteintech
SKU: 11891-1-AP
Rating: 4.00 (1 reviews)

Prealbumin/transthyretin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11891-1-AP

Synonyms

Prealbumin, transthyretin, TTR, TBPA, PALB

Validation Data Gallery

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AllWBIHCIF-P

WB analysis using 11891-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11891-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human spleen using 11891-1-AP
human spleen tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11891-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11891-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11891-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11891-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11891-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11891-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11891-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11891-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 11891-1-AP
human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 11891-1-AP
human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human plasma using 11891-1-AP
human blood were subjected to SDS PAGE followed by western blot with 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 11891-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver cancer using 11891-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 11891-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11891-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11891-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11891-1-AP (Prealbumin/transthyretin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 11891-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Prealbumin/transthyretin antibody (11891-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11891-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human plasma, human brain tissue, human heart tissue, HEK-293 cells, human spleen tissue, human liver tissue, human serum
Positive IHC detected in	human liver cancer tissue, mouse liver tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 7 publications below
IF	See 4 publications below

Product Information

11891-1-AP targets Prealbumin/transthyretin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Prealbumin/transthyretin fusion protein Ag2499 相同性解析による交差性が予測される生物種
Full Name	transthyretin
Calculated molecular weight	147 aa, 16 kDa
Observed molecular weight	16 kDa
GenBank accession number	BC020791
Gene symbol	TTR
Gene ID (NCBI)	7276
RRID	AB_2212083
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)

Protocols

Product Specific Protocols
WB protocol for Prealbumin/transthyretin antibody 11891-1-AP	Download protocol
IHC protocol for Prealbumin/transthyretin antibody 11891-1-AP	Download protocol
IF protocol for Prealbumin/transthyretin antibody 11891-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	IF	Cell Ex utero monkey embryogenesis from blastocyst to early organogenesis Authors - Yandong Gong View Article
mouse	IHC	Dev Cell Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS. Authors - Alison A Lanctot View Article
human	IF	Dev Cell Lysosomal dynamics regulate mammalian cortical neurogenesis Authors - Wenzheng Zou View Article
mouse	WB	Theranostics Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4. Authors - Jinxin Liu View Article
	WB	Diabetes PMID: 25524914 Authors - Laura Zemany View Article
human	WB	Int J Mol Sci Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest. Authors - Marta Zarà View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Kenzo (Verified Customer) (11-11-2023)

This antibody worked for mouse heart tissue sections with little background.

Applications: Immunofluorescence
Primary Antibody Dilution: 1:100
Cell Tissue Type: Heart

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Prealbumin/transthyretin Polyclonal antibody

Prealbumin/transthyretin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 11891-1-AP

WB analysis of human liver using 11891-1-AP

WB analysis of human spleen using 11891-1-AP

WB analysis of human liver using 11891-1-AP

WB analysis of human brain using 11891-1-AP

WB analysis of human heart using 11891-1-AP

WB analysis of HEK-293 using 11891-1-AP

WB analysis of human brain using 11891-1-AP

WB analysis of human brain using 11891-1-AP

WB analysis of human brain using 11891-1-AP

WB analysis of human brain using 11891-1-AP

WB analysis of human plasma using 11891-1-AP

WB analysis of human plasma using 11891-1-AP

WB analysis of human plasma using 11891-1-AP

IHC staining of human liver cancer using 11891-1-AP

IHC staining of human liver cancer using 11891-1-AP

IHC staining of human kidney using 11891-1-AP

IHC staining of mouse liver using 11891-1-AP

IHC staining of mouse liver using 11891-1-AP

IF Staining of mouse brain using 11891-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ex utero monkey embryogenesis from blastocyst to early organogenesis

Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS.

Lysosomal dynamics regulate mammalian cortical neurogenesis

Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.

PMID: 25524914

Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest.

Reviews