Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, HEK-293 cells, human brain tissue, human heart tissue, human liver tissue, human spleen tissue, human serum |
| Positive IHC detected in | mouse colon tissue, mouse heart tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 7 publications below |
| IF | See 5 publications below |
Product Information
11891-1-AP targets Prealbumin/transthyretin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Prealbumin/transthyretin fusion protein Ag2499 相同性解析による交差性が予測される生物種 |
| Full Name | transthyretin |
| Calculated molecular weight | 147 aa, 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC020791 |
| Gene Symbol | TTR |
| Gene ID (NCBI) | 7276 |
| RRID | AB_2212083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Prealbumin/transthyretin antibody 11891-1-AP | Download protocol |
| IHC protocol for Prealbumin/transthyretin antibody 11891-1-AP | Download protocol |
| IF protocol for Prealbumin/transthyretin antibody 11891-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Spatially dependent dynamic MAPK modulation by the Nde1-Lis1-Brap complex patterns mammalian CNS. | ||
Theranostics Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4. | ||
Int J Mol Sci Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest. |