Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, human spleen tissue, HeLa cells |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
Product Information
12203-1-AP targets TTRAP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TTRAP fusion protein Ag2846 相同性解析による交差性が予測される生物種 |
| Full Name | TRAF and TNF receptor associated protein |
| Calculated molecular weight | 362 aa, 41 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC017553 |
| Gene Symbol | TTRAP |
| Gene ID (NCBI) | 51567 |
| RRID | AB_1607504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95551 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TTRAP is a 5-prime tyrosyl-DNA phosphodiesterase that is required for efficient repair of topoisomerase II-induced DNA double-strand breaks. Thus it can remove lots of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, generating a free 5' phosphate to DNA. It also has 3'-tyrosyl DNA phophodiesterase activity, but not so efficient as TDP1. TTRAP functions as a negative regulator of ETS1 and inhibit nuclear factor-kappa-B activation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TTRAP antibody 12203-1-AP | Download protocol |
| IP protocol for TTRAP antibody 12203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2. | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.
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Anal Biochem Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase. | ||
Int J Mol Sci Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer | ||
bioRxiv DNA polymerase theta-mediated DNA repair is a functional dependency and therapeutic vulnerability in DNMT3A deficient leukemia cells |