Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 14555-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 14555-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14555-1-AP (alpha Tubulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 14555-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14555-1-AP (Alpha Tubulin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14555-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HeLa using 14555-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (14555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14555-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TUBA1A, Tubulin alpha 1A chain, Alpha-tubulin 3, Detyrosinated tubulin alpha-1A chain, EC:3.6.5.-

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (18)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse cerebellum tissue, HeLa cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue, rat kidney tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 17 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

14555-1-AP targets Alpha Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Alpha Tubulin fusion protein Ag6038 相同性解析による交差性が予測される生物種
Full Name	tubulin, alpha 1a
Calculated molecular weight	50 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC050637
Gene Symbol	Alpha Tubulin
Gene ID (NCBI)	7846
RRID	AB_2212258
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q71U36
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 14555-1-AP	Download protocol
IHC protocol for Alpha Tubulin antibody 14555-1-AP	Download protocol
IF protocol for Alpha Tubulin antibody 14555-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
bovine	WB	Int J Biol Macromol NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells Authors - Wanghao Yang View Article
human	WB	mBio Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner Authors - Xia-Ming Jiang View Article
mouse	WB	J Neurosci Res Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion. Authors - Shaqayeq Roqanian View Article
human	WB	J Cell Sci ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments. Authors - Mohamed Bourmoum View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E. Authors - Yan Wang View Article
human	WB,IF	Exp Cell Res Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression. Authors - Shen Enzhi E View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14555-1-AP

WB analysis of mouse cerebellum using 14555-1-AP

IHC staining of mouse testis using 14555-1-AP

IF Staining of HeLa using 14555-1-AP

IF Staining of HeLa using 14555-1-AP

Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

NR1D1 activation alleviates inflammatory response through inhibition of IL-6 expression in bovine endometrial epithelial cells

Regulation of the WNT-CTNNB1 signaling pathway by severe fever with thrombocytopenia syndrome virus in a cap-snatching manner

Tau nuclear translocation is a leading step in tau pathology process through P53 stabilization and nucleolar dispersion.

ARF6 protects sister chromatid cohesion to ensure the formation of stable kinetochore-microtubule attachments.

Dicer1 promotes Aβ clearance via blocking B2 RNA-mediated repression of apolipoprotein E.

Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression.