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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16007-1-AP

Synonyms

alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Tubulin Polyclonal antibody (16007-1-AP)

    WB analysis using 16007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat eye tissue using Alpha Tubulin Polyclonal antibody (16007-1-AP)

    IHC staining of rat eye using 16007-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, zebrafish tissues, mouse ovary tissues
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16007-1-AP targets Alpha Tubulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

Tested Reactivity human, mouse, zebrafish, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag8695 相同性解析による交差性が予測される生物種
Full Name tubulin, alpha 1c
Calculated molecular weight 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC004949
Gene symbol Alpha Tubulin
Gene ID (NCBI) 84790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols