Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis of HeLa using 15176-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 15176-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15176-1-AP (gamma Tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15176-1-AP
IP result of anti-Gamma Tubulin (IP:15176-1-AP, 4ug; Detection:15176-1-AP 1:2000) with HeLa cells lysate 1600ug.
IHC staining of human prostate cancer using 15176-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 15176-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 15176-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 10x lens).
IHC staining of human prostate hyperplasia using 15176-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15176-1-AP (gamma Tubulin antibody at dilution of 1:400 (under 40x lens).
FC experiment of HeLa using 15176-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (15176-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 15176-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, Transfected HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue, human prostate hyperplasia tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 3 publications below
IF	See 17 publications below

Product Information

15176-1-AP targets Gamma Tubulin in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Gamma Tubulin fusion protein Ag7070 相同性解析による交差性が予測される生物種
Full Name	tubulin, gamma 1
Calculated molecular weight	51 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC000619
Gene Symbol	Gamma Tubulin
Gene ID (NCBI)	7283
RRID	AB_2878113
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P23258
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Protocols

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 15176-1-AP	Download protocol
IHC protocol for Gamma Tubulin antibody 15176-1-AP	Download protocol
IP protocol for Gamma Tubulin antibody 15176-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cancer Cell Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2. Authors - Shihao Zhang View Article
human	IF,WB	Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex Authors - Lauren G. Mascibroda View Article
mouse	IF	PLoS Biol Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins. Authors - Suchismita Panda View Article
mouse	WB	Cell Death Differ Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina. Authors - Zhigang Li View Article
human	IF	Br J Pharmacol NL13, a novel curcumin analogue and polo like kinase 4 inhibitor, induces cell cycle arrest and apoptosis in prostate cancer models Authors - Xinyi Qiao View Article
human	WB	Elife LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome. Authors - Laura Bozal-Basterra View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15176-1-AP

WB analysis of Transfected HEK-293 using 15176-1-AP

IP experiment of HeLa using 15176-1-AP

IHC staining of human prostate cancer using 15176-1-AP

IHC staining of human prostate cancer using 15176-1-AP

IHC staining of human prostate hyperplasia using 15176-1-AP

IHC staining of human prostate hyperplasia using 15176-1-AP

FC experiment of HeLa using 15176-1-AP

Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hippo Signaling Suppresses Cell Ploidy and Tumorigenesis through Skp2.

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex

Noncoding RNA Ginir functions as an oncogene by associating with centrosomal proteins.

Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.

NL13, a novel curcumin analogue and polo like kinase 4 inhibitor, induces cell cycle arrest and apoptosis in prostate cancer models

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.