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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TUBGCP4 Polyclonal antibody

TUBGCP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17088-1-AP

Synonyms

76P, GCP 4, GCP4, h76p, hGCP4, hGrip76, TUBGCP4

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TUBGCP4 Polyclonal antibody (17088-1-AP)

    WB analysis of MCF-7 using 17088-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17088-1-AP (TUBGCP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TUBGCP4 Polyclonal antibody (17088-1-AP)

    WB analysis of HepG2 using 17088-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17088-1-AP (TUBGCP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TUBGCP4 Polyclonal antibody (17088-1-AP)

    IHC staining of human placenta using 17088-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17088-1-AP (TUBGCP4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TUBGCP4 Polyclonal antibody (17088-1-AP)

    IHC staining of human placenta using 17088-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17088-1-AP (TUBGCP4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17088-1-AP targets TUBGCP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUBGCP4 fusion protein Ag9976 相同性解析による交差性が予測される生物種
Full Name tubulin, gamma complex associated protein 4
Calculated molecular weight 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC000966
Gene symbol TUBGCP4
Gene ID (NCBI) 27229
RRIDAB_2211283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TUBGCP4 antibody 17088-1-APDownload protocol
IHC protocol for TUBGCP4 antibody 17088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols