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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TUFM Monoclonal antibody (67802-1-Ig)

    WB analysis using 67802-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TUFM Monoclonal antibody (67802-1-Ig)

    WB analysis using 67802-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TUFM Monoclonal antibody (67802-1-Ig)

    IP experiment of HepG2 using 67802-1-Ig

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TUFM Monoclonal antibody (67802-1-Ig)

    IHC staining of human colon cancer using 67802-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TUFM Monoclonal antibody (67802-1-Ig)

    IHC staining of human colon cancer using 67802-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TUFM Monoclonal antibody

TUFM Monoclonal Antibody for WB, IHC, IP, ELISA
Cat No. 67802-1-Ig
Clone No.2A10D2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HCT 116 cells, HepG2 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, 4T1 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67802-1-Ig targets TUFM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TUFM fusion protein Ag24976 相同性解析による交差性が予測される生物種
Full Name Tu translation elongation factor, mitochondrial
Calculated molecular weight 50 kDa
Observed molecular weight48-50 kDa
GenBank accession numberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
RRIDAB_2918565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49411
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protocols

Product Specific Protocols
WB protocol for TUFM antibody 67802-1-IgDownload protocol
IHC protocol for TUFM antibody 67802-1-IgDownload protocol
IP protocol for TUFM antibody 67802-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols