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  • Featured Product
  • KD/KO Validated

TUFT1 Polyclonal antibody

TUFT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23385-1-AP

Synonyms

tuftelin1, tuftelin 1, Tuftelin

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TUFT1 Polyclonal antibody (23385-1-AP)

    WB analysis of HEK-293 using 23385-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TUFT1 Polyclonal antibody (23385-1-AP)

    WB analysis of HeLa using 23385-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TUFT1 Polyclonal antibody (23385-1-AP)

    WB analysis of HEK-293 using 23385-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TUFT1 Polyclonal antibody (23385-1-AP)

    WB analysis of HEK-293 using 23385-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TUFT1 Polyclonal antibody (23385-1-AP)

    IHC staining of human testis using 23385-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23385-1-AP (TUFT1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TUFT1 Polyclonal antibody (23385-1-AP)

    IF Staining of HEK-293 using 23385-1-AP

    Immunofluorescent analysis of HEK-293 cells using 23385-1-AP (TUFT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23385-1-AP targets TUFT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUFT1 fusion protein Ag20020 相同性解析による交差性が予測される生物種
Full Name tuftelin 1
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC002933
Gene symbol TUFT1
Gene ID (NCBI) 7286
RRIDAB_2879270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TUFT1 antibody 23385-1-APDownload protocol
IHC protocol for TUFT1 antibody 23385-1-APDownload protocol
IF protocol for TUFT1 antibody 23385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pathol Oncol Res

Tuftelin 1 (TUFT1) Promotes the Proliferation and Migration of Renal Cell Carcinoma via PI3K/AKT Signaling Pathway.

Authors - Hua Lin
  • KD Validated
View Article