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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TWEAKR/CD266 Polyclonal antibody

TWEAKR/CD266 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 19836-1-AP

Synonyms

CD266, TNFRSF12A, TWEAKR, FGF-inducible 14, FGF inducible 14

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using TWEAKR Polyclonal antibody (19836-1-AP)

    IHC staining of mouse heart using 19836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19836-1-AP (TWEAKR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TWEAKR Polyclonal antibody (19836-1-AP)

    IHC staining of mouse heart using 19836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19836-1-AP (TWEAKR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19836-1-AP targets TWEAKR/CD266 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWEAKR/CD266 fusion protein Ag13823 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 12A
Calculated molecular weight 129 aa, 14 kDa
GenBank accession numberBC002718
Gene symbol TWEAKR
Gene ID (NCBI) 51330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)

Protocols

Product Specific Protocols
IHC protocol for TWEAKR/CD266 antibody 19836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols