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KD/KO Validated

TWF1 Polyclonal antibody

Name: TWF1 Polyclonal antibody
Brand: Proteintech
SKU: 11732-1-AP

TWF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11732-1-AP

Synonyms

Twinfilin-1, Twinfilin 1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 11732-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 11732-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11732-1-AP (TWF1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11732-1-AP
WB result of TWF1 antibody (11732-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TWF1 transfected HeLa cells.
IHC staining of human lung cancer using 11732-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11732-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11732-1-AP (TWF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11732-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TWF1 antibody (11732-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11732-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C6 cells, HeLa cells, C2C12 cells, mouse testis tissue, rat testis tissue, NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

11732-1-AP targets TWF1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TWF1 fusion protein Ag2332 相同性解析による交差性が予測される生物種
Full Name	twinfilin, actin-binding protein, homolog 1 (Drosophila)
Calculated molecular weight	368 aa, 42 kDa
Observed molecular weight	42 kDa
GenBank accession number	BC022344
Gene symbol	TWF1
Gene ID (NCBI)	5756
RRID	AB_2241259
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TWF1 antibody 11732-1-AP	Download protocol
IHC protocol for TWF1 antibody 11732-1-AP	Download protocol
IF protocol for TWF1 antibody 11732-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Biochem Biophys Res Commun Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway. Authors - Lu Li KD Validated View Article
human	WB	J Thorac Dis Overexpression of TWF1 promotes lung adenocarcinoma progression and is associated with poor prognosis in cancer patients through the MMP1 signaling pathway Authors - Kui Zhai KD Validated View Article
human,mouse	WB,IHC,CoIP	FASEB J TWF1 induces autophagy and accelerates malignant phenotype in lung adenocarcinoma via inhibiting the cAMP signaling pathway Authors - Yu Wang KD Validated View Article
mouse	WB	Sci Signal The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex Authors - Sharof Khudayberdiev View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TWF1 Polyclonal antibody

TWF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 11732-1-AP

WB analysis using 11732-1-AP

WB analysis of HeLa using 11732-1-AP

IHC staining of human lung cancer using 11732-1-AP

IHC staining of human breast cancer using 11732-1-AP

IF Staining of HeLa using 11732-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway.

Overexpression of TWF1 promotes lung adenocarcinoma progression and is associated with poor prognosis in cancer patients through the MMP1 signaling pathway

TWF1 induces autophagy and accelerates malignant phenotype in lung adenocarcinoma via inhibiting the cAMP signaling pathway

The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex