TWIST1 Polyclonal antibody
TWIST1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18125-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-3 cells, MCF-7 cells, SGC-7901 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 17 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 18125-1-AP is TWIST1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TWIST1 fusion protein Ag12700 相同性解析による交差性が予測される生物種 |
Full Name | twist homolog 1 (Drosophila) |
Calculated molecular weight | 202 aa, 21 kDa |
Observed molecular weight | 22 kDa, 28 kDa, 45-60 kDa |
GenBank accession number | BC036704 |
Gene symbol | TWIST1 |
Gene ID (NCBI) | 7291 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).
Protocols
Product Specific Protocols | |
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WB protocol for TWIST1 antibody 18125-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis. | ||
Int J Biochem Cell Biol Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage. | ||
Cancer Lett Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry. | ||
Int J Cancer Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer. | ||
Sci Rep Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model. |