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  • Featured Product
  • KD/KO Validated

TWIST1-specific Polyclonal antibody

TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 25465-1-AP

Synonyms

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1, TWIST1-specific

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TWIST1-specific Polyclonal antibody (25465-1-AP)

    WB analysis using 25465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using TWIST1-specific Polyclonal antibody (25465-1-AP)

    WB analysis of SH-SY5Y using 25465-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using TWIST1-specific Polyclonal antibody (25465-1-AP)

    WB analysis of Raji using 25465-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TWIST1-specific Polyclonal antibody (25465-1-AP)

    WB analysis of A549 using 25465-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MDA-MB-453s cells using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IP experiment of MDA-MB-453s using 25465-1-AP

    IP result of anti-TWIST1-specific (IP:25465-1-AP, 4ug; Detection:25465-1-AP 1:500) with MDA-MB-453s cells lysate 920ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IHC staining of human liver cancer using 25465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IHC staining of human pancreas cancer using 25465-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IHC staining of human breast cancer using 25465-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IHC staining of human breast cancer using 25465-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IHC staining of human pancreas cancer using 25465-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IHC staining of human liver cancer using 25465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using TWIST1-specific Polyclonal antibody (25465-1-AP)

    IF Staining of L02 using 25465-1-AP

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using TWIST1-specific antibody (25465-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inU-251 cells, Raji cells, A549 cells, SH-SY5Y cells, MDA-MB-231 cells, PC-3 cells
Positive IP detected inMDA-MB-453s cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25465-1-AP targets TWIST1-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWIST1-specific fusion protein Ag21924 相同性解析による交差性が予測される生物種
Full Name twist homolog 1 (Drosophila)
Calculated molecular weight 202 aa, 21 kDa
Observed molecular weight25-30 kDa
GenBank accession numberBC036704
Gene symbol TWIST1
Gene ID (NCBI) 7291
RRIDAB_2880093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Protocols

Product Specific Protocols
WB protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IHC protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IF protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IP protocol for TWIST1-specific antibody 25465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.

Authors - Yi Wu
View Article
mouseWB,IHC

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin
View Article
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
View Article
mouseWB,IHC

Int J Biol Macromol

Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression

Authors - Yu-Wei Yen
View Article
humanWB,IHC

Int J Nanomedicine

Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model

Authors - Guoqiang Xu
View Article
humanIHC

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Pramod Bahadur (Verified Customer) (02-27-2024)

We are missing the vials so I dont have lot number.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC3
TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in PC3 (Cat no:25465-1-AP)