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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using TWIST2 Polyclonal antibody (11752-1-AP)

    WB analysis of NIH/3T3 using 11752-1-AP (same clone as 11752-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using TWIST2 Polyclonal antibody (11752-1-AP)

    WB analysis of NIH/3T3 using 11752-1-AP (same clone as 11752-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TWIST2 Polyclonal antibody (11752-1-AP)

    WB analysis of mouse liver using 11752-1-AP (same clone as 11752-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11752-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TWIST2 Polyclonal antibody (11752-1-AP)

    WB analysis of MCF-7 using 11752-1-AP (same clone as 11752-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11752-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TWIST2 Polyclonal antibody, PBS Only

TWIST2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 11752-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11752-1-PBS targets TWIST2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWIST2 fusion protein Ag2348 相同性解析による交差性が予測される生物種
Full Name twist homolog 2 (Drosophila)
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight 21 kDa, 60 kDa
GenBank accession numberBC033168
Gene Symbol TWIST2
Gene ID (NCBI) 117581
RRIDAB_2877791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVJ9
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).