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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TWIST2 Monoclonal antibody (66544-1-Ig)

    WB analysis using 66544-1-Ig (same clone as 66544-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66544-1-Ig (TWIST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TWIST2 Monoclonal antibody (66544-1-Ig)

    IHC staining of human breast cancer using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TWIST2 Monoclonal antibody (66544-1-Ig)

    IHC staining of human ovary tumor using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TWIST2 Monoclonal antibody (66544-1-Ig)

    IHC staining of human ovary tumor using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TWIST2 Monoclonal antibody (66544-1-Ig)

    IHC staining of human breast cancer using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66544-1-Ig (TWIST2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TWIST2 Monoclonal antibody (66544-1-Ig)

    IF Staining of HepG2 using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TWIST2 Monoclonal antibody (66544-1-Ig)

    IF Staining of HepG2 using 66544-1-Ig (same clone as 66544-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TWIST2 antibody (66544-1-Ig, Clone: 1B12D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66544-1-PBS in a different storage buffer formulation.

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TWIST2 Monoclonal antibody, PBS Only

TWIST2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66544-1-PBS
Clone No.1B12D11

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66544-1-PBS targets TWIST2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Reactivity Human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TWIST2 fusion protein Ag2348 相同性解析による交差性が予測される生物種
Full Name twist homolog 2 (Drosophila)
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC033168
Gene Symbol TWIST2
Gene ID (NCBI) 117581
RRIDAB_2881906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WVJ9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).