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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TWISTNB Polyclonal antibody

TWISTNB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22784-1-AP

Synonyms

TWIST neighbor, RPA43, RNA polymerase I subunit F, POLR1F, DNA-directed RNA polymerase I subunit RPA43

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TWISTNB Polyclonal antibody (22784-1-AP)

    WB analysis of HeLa using 22784-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using TWISTNB Polyclonal antibody (22784-1-AP)

    WB analysis of THP-1 using 22784-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TWISTNB Polyclonal antibody (22784-1-AP)

    WB analysis using 22784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TWISTNB Polyclonal antibody (22784-1-AP)

    IHC staining of human ovary cancer using 22784-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22784-1-AP (TWISTNB antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22784-1-AP targets TWISTNB in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWISTNB fusion protein Ag18783 相同性解析による交差性が予測される生物種
Full Name TWIST neighbor
Calculated molecular weight 338 aa, 37 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC130298
Gene symbol TWISTNB
Gene ID (NCBI) 221830
RRIDAB_2879167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TWISTNB antibody 22784-1-APDownload protocol
IHC protocol for TWISTNB antibody 22784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols