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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TXK Polyclonal antibody

TXK Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18981-1-AP

Synonyms

PTK4, PSCTK5, Protein-tyrosine kinase 4, Protein tyrosine kinase 4, EC:2.7.10.2

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TXK Polyclonal antibody (18981-1-AP)

    IHC staining of human lymphoma using 18981-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18981-1-AP (TXK antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TXK Polyclonal antibody (18981-1-AP)

    IHC staining of human lymphoma using 18981-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18981-1-AP (TXK antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TXK Polyclonal antibody (18981-1-AP)

    IF Staining of HeLa using 18981-1-AP

    Immunofluorescent analysis of Hela cells, using TXK antibody 18981-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TXK Polyclonal antibody (18981-1-AP)

    IF Staining of HepG2 using 18981-1-AP

    Immunofluorescent analysis of HepG2 cells, using TXK antibody 18981-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

18981-1-AP targets TXK in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name TXK tyrosine kinase
Calculated molecular weight 61 kDa
GenBank accession numberNM_003328
Gene symbol TXK
Gene ID (NCBI) 7294
RRIDAB_10598482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXK, also named as PTK4, is an enzyme that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.

Protocols

Product Specific Protocols
IHC protocol for TXK antibody 18981-1-APDownload protocol
IF protocol for TXK antibody 18981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biomedicines

Establishing Molecular Subgroups of CD8+ T Cell-Associated Genes in the Ovarian Cancer Tumour Microenvironment and Predicting the Immunotherapy Response

Authors - Yunshu Zhu
View Article