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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ERp19 Polyclonal antibody (20181-1-AP)

    WB analysis of human brain using 20181-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using ERp19 Polyclonal antibody (20181-1-AP)

    WB analysis of human heart using 20181-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ERp19 Polyclonal antibody (20181-1-AP)

    WB analysis of mouse lung using 20181-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ERp19 Polyclonal antibody (20181-1-AP)

    WB analysis of mouse kidney using 20181-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERp19 Polyclonal antibody (20181-1-AP)

    IHC staining of human liver cancer using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERp19 Polyclonal antibody (20181-1-AP)

    IHC staining of human liver cancer using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ERp19 Polyclonal antibody (20181-1-AP)

    IHC staining of human hepatocirrhosis using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ERp19 Polyclonal antibody (20181-1-AP)

    IHC staining of human hepatocirrhosis using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ERp19 Polyclonal antibody (20181-1-AP)

    IHC staining of human hepatocirrhosis using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ERp19 Polyclonal antibody (20181-1-AP)

    IHC staining of human hepatocirrhosis using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20181-1-AP (ERp19 antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ERp19 Polyclonal antibody

ERp19 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20181-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue, mouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20181-1-AP targets ERp19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp19 fusion protein Ag14045 相同性解析による交差性が予測される生物種
Full Name thioredoxin domain containing 12 (endoplasmic reticulum)
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 20 kDa, 40 kDa
GenBank accession numberBC001493
Gene Symbol TXNDC12
Gene ID (NCBI) 51060
RRIDAB_10646468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95881
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXNDC12, which is known as thioredoxin domain-containing protein 12, ERp16, ERp18, ERp19, or hTLP19, is a member of the protein disulfide isomerase (PDI) family that plays important roles in cancer development and progression. TXNDC12 has been demonstrated to contribute to tumorigenicity in human gastric cancer by promoting cell growth, migration, and invasion(PMID: 25940440). Other studies also revealed the overexpression of TXNDC12 promotes HCC metastasis through β-catenin-mediated induction of EMT and that TXNDC12 has the potential to be a prognostic factor and therapeutic target of HCC. (PMID: 31570854)

Protocols

Product Specific Protocols
WB protocol for ERp19 antibody 20181-1-APDownload protocol
IHC protocol for ERp19 antibody 20181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols