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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis using 20811-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of Jurkat using 20811-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of HEK-293 using 20811-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of HeLa using 20811-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of mouse pancreas using 20811-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human uterus tissue using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of human uterus using 20811-1-AP

    human uterus tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of K-562 using 20811-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of human kidney using 20811-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    WB analysis of L02 using 20811-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IP experiment of HEK-293 using 20811-1-AP

    IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:6000) with HEK-293 cells lysate 1575 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IF Staining of HepG2 using 20811-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TXNDC17 antibody (20811-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IF Staining of HeLa using 20811-1-AP

    Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TXNDC17 Polyclonal antibody (20811-1-AP)

    IF Staining of HepG2 using 20811-1-AP

    Immunofluorescent analysis of HepG2 cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

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TXNDC17 Polyclonal antibody

TXNDC17 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 20811-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, human kidney tissue, human uterus tissue, Jurkat cells, K-562 cells, L02 cells, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20811-1-AP targets TXNDC17 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNDC17 fusion protein Ag14766 相同性解析による交差性が予測される生物種
Full Name thioredoxin domain containing 17
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC006405
Gene Symbol TXNDC17
Gene ID (NCBI) 84817
RRIDAB_10695499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXNDC17 also known as TRP14, TXNL5, belongs to the thioredoxin family. TRP14 is a widely expressed, 123-amino acid protein with a WCPDC motif. TXNDC17 has a thioredoxin fold and a TRX-like active-site sequence(PMID: 14607844, 24778250).

Protocols

Product Specific Protocols
WB protocol for TXNDC17 antibody 20811-1-APDownload protocol
IF protocol for TXNDC17 antibody 20811-1-APDownload protocol
IP protocol for TXNDC17 antibody 20811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Radiat Biol

Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice.

Authors - Sania Bajaj
View Article