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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TXNDC5 Monoclonal antibody

TXNDC5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12C3

Cat no : 68609-1-Ig

Synonyms

EndoPDI, ER protein 46, ERP46, Hcc 2, PDIA15, Thioredoxin like protein p46, TLP46, TXNDC5, UNQ364

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TXNDC5 Monoclonal antibody (68609-1-Ig)

    WB analysis using 68609-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68609-1-Ig (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of mouse colon tissue using TXNDC5 Monoclonal antibody (68609-1-Ig)

    WB analysis of mouse colon using 68609-1-Ig

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 68609-1-Ig (TXNDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, mouse colon tissue, COLO 320 cells, HepG2 cells, LNCaP cells, Jurkat cells, K-562 cells, TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68609-1-Ig targets TXNDC5 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TXNDC5 fusion protein Ag13965 相同性解析による交差性が予測される生物種
Full Name thioredoxin domain containing 5 (endoplasmic reticulum)
Calculated molecular weight 432 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC001199
Gene symbol TXNDC5
Gene ID (NCBI) 81567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TXNDC5 antibody 68609-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

TXNDC5 Plays a Crucial Role in Regulating Endoplasmic Reticulum Activity through Different ER Stress Signaling Pathways in Hepatic Cells

Authors - Seyed Hesamoddin Bidooki
View Article