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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TXNDC9 Monoclonal antibody (68335-1-Ig)

    WB analysis of HEK-293 using 68335-1-Ig (same clone as 68335-1-PBS)

    WB result of TXNDC9 antibody (68335-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNDC9 transfected HEK-293 cells. This data was developed using the same antibody clone with 68335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TXNDC9 Monoclonal antibody (68335-1-Ig)

    WB analysis using 68335-1-Ig (same clone as 68335-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68335-1-Ig (TXNDC9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68335-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using TXNDC9 Monoclonal antibody (68335-1-Ig)

    FC experiment of HEK-293 using 68335-1-Ig (same clone as 68335-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug TXNDC9 Monoclonal antibody (68335-1-Ig, Clone:2A10G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68335-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

TXNDC9 Monoclonal antibody, PBS Only

TXNDC9 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68335-1-PBS
Clone No.2A10G3

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Synonyms

Thioredoxin domain-containing protein 9, Protein 1-4, Protein 1 4, PHLP3, ATP-binding protein associated with cell differentiation

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68335-1-PBS targets TXNDC9 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TXNDC9 fusion protein Ag33259 相同性解析による交差性が予測される生物種
Full Name thioredoxin domain containing 9
Calculated molecular weight 226 aa, 27 kDa
Observed molecular weight27 kDa
GenBank accession numberBC005968
Gene Symbol TXNDC9
Gene ID (NCBI) 10190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14530
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TXNDC9, also known as APACD, has a negative effect on protein folding by significantly reducing the activity of chaperone protein TCP1 complex ATPase activity. Including actin or tubulin.