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TXNRD1 Polyclonal antibody

Name: TXNRD1 Polyclonal antibody
Brand: Proteintech
SKU: 11117-1-AP

TXNRD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11117-1-AP

Synonyms

TRXR1, GRIM12, GRIM 12, Gene associated with retinoic and interferon-induced mortality 12 protein, Gene associated with retinoic and IFN-induced mortality 12 protein

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 11117-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11117-1-AP
WB result of TXNRD1 antibody (11117-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNRD1 transfected HeLa cells.
WB analysis of MCF-7 using 11117-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11117-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11117-1-AP (TXNRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 11117-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11117-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11117-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11117-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11117-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11117-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11117-1-AP (TXNRD1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11117-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNRD1 antibody (11117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 11117-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IHC detected in	human testis tissue, human breast cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:3000-1:10000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 51 publications below
IHC	See 9 publications below
IF	See 4 publications below

Product Information

11117-1-AP targets TXNRD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TXNRD1 fusion protein Ag1618 相同性解析による交差性が予測される生物種
Full Name	thioredoxin reductase 1
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC018122
Gene symbol	TXNRD1
Gene ID (NCBI)	7296
RRID	AB_2210113
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Protocols

Product Specific Protocols
WB protocol for TXNRD1 antibody 11117-1-AP	Download protocol
IHC protocol for TXNRD1 antibody 11117-1-AP	Download protocol
IF protocol for TXNRD1 antibody 11117-1-AP	Download protocol
FC protocol for TXNRD1 antibody 11117-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Redox Biol Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation. Authors - Angel Gallego-Selles View Article
human,mouse	WB	J Immunother Cancer Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis. Authors - Weinan Guo View Article
human	WB	Cell Death Dis Elovanoid-N34 modulates TXNRD1 key in protection against oxidative stress-related diseases Authors - Jorgelina M Calandria View Article
human	WB	Cancer Res Natural allelic variations in glutathione peroxidase-1 affect it subcellular localization and function. Authors - Soumen Bera View Article
rat	WB	Nucleic Acids Res Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression. Authors - Miniard Angela C AC View Article
human	WB	Biomed Pharmacother TrxR/Trx inhibitor butaselen ameliorates pulmonary fibrosis by suppressing NF-κB/TGF-β1/Smads signaling Authors - Yifan Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TXNRD1 Polyclonal antibody

TXNRD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 11117-1-AP

WB analysis of HeLa using 11117-1-AP

WB analysis of MCF-7 using 11117-1-AP

WB analysis of Jurkat using 11117-1-AP

IHC staining of human testis using 11117-1-AP

IHC staining of human breast cancer using 11117-1-AP

IHC staining of human breast cancer using 11117-1-AP

IHC staining of mouse testis using 11117-1-AP

IHC staining of mouse testis using 11117-1-AP

IHC staining of mouse testis using 11117-1-AP

IF Staining of HeLa using 11117-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.

Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.

Elovanoid-N34 modulates TXNRD1 key in protection against oxidative stress-related diseases

Natural allelic variations in glutathione peroxidase-1 affect it subcellular localization and function.

Nucleolin binds to a subset of selenoprotein mRNAs and regulates their expression.

TrxR/Trx inhibitor butaselen ameliorates pulmonary fibrosis by suppressing NF-κB/TGF-β1/Smads signaling