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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TYK2 Polyclonal antibody

TYK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16412-1-AP

Synonyms

tyrosine kinase 2, JTK1, EC:2.7.10.2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TYK2 Polyclonal antibody (16412-1-AP)

    WB analysis using 16412-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16412-1-AP (TYK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TYK2 Polyclonal antibody (16412-1-AP)

    IHC staining of human liver cancer using 16412-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16412-1-AP (TYK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TYK2 Polyclonal antibody (16412-1-AP)

    IHC staining of human liver cancer using 16412-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16412-1-AP (TYK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TYK2 Polyclonal antibody (16412-1-AP)

    IF Staining of A549 using 16412-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TYK2 antibody (16412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16412-1-AP targets TYK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TYK2 fusion protein Ag9462 相同性解析による交差性が予測される生物種
Full Name tyrosine kinase 2
Calculated molecular weight 1187 aa, 134 kDa
Observed molecular weight134 kDa
GenBank accession numberBC014243
Gene symbol TYK2
Gene ID (NCBI) 7297
RRIDAB_2918047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TYK2 antibody 16412-1-APDownload protocol
IHC protocol for TYK2 antibody 16412-1-APDownload protocol
IF protocol for TYK2 antibody 16412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

JAK1/JAK2 degraders based on PROTAC for topical treatment of atopic dermatitis

Authors - Junchao Wu
View Article
mouseWB

Dev Cell

Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression

Authors - Cheng Zhang
View Article