TYK2 Monoclonal antibody

Name: TYK2 Monoclonal antibody
Brand: Proteintech
SKU: 67411-1-Ig

TYK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

1E1A10

Review Product

Cat no : 67411-1-Ig

Synonyms

JTK1, TYK2, tyrosine kinase 2

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 67411-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis using 67411-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 67411-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
IHC staining of human colon cancer using 67411-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67411-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67411-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67411-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67411-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67411-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67411-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (67411-1-Ig, Clone: 1E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Cat No. 67411-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, K-562 cells, MJ cells, Jurkat cells, MOLT-4 cells, TF-1 cells, HSC-T6 cells, A549 cells, THP-1 cells
Positive IHC detected in	human colon cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below

Product Information

67411-1-Ig targets TYK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity	Human, rat
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	TYK2 fusion protein Ag9442 相同性解析による交差性が予測される生物種
Full Name	tyrosine kinase 2
Calculated molecular weight	1187 aa, 134 kDa
Observed molecular weight	134 kDa
GenBank accession number	BC014243
Gene symbol	TYK2
Gene ID (NCBI)	7297
RRID	AB_2882652
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TYK2 antibody 67411-1-Ig	Download protocol
IHC protocol for TYK2 antibody 67411-1-Ig	Download protocol
IF protocol for TYK2 antibody 67411-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Virol SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway. Authors - Da-Yuan Chen View Article
human	WB	Invest New Drugs IL-10 contributes to gemcitabine resistance in extranodal NK/T-cell lymphoma cells via ABCC4. Authors - Jia Huo View Article
human	WB	bioRxiv SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway. Authors - Da-Yuan Chen View Article
human	WB	Cancer Lett HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma Authors - Xueshuo Sun View Article
mouse	WB	Antioxidants (Basel) Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I Interferon Responses Authors - Shuni Duan View Article
mouse	WB	Chin J Nat Med Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses Authors - Kexin Jia View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TYK2 Monoclonal antibody

TYK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 67411-1-Ig

WB analysis using 67411-1-Ig

WB analysis using 67411-1-Ig

IHC staining of human colon cancer using 67411-1-Ig

IHC staining of human colon cancer using 67411-1-Ig

IHC staining of human liver cancer using 67411-1-Ig

IHC staining of human liver cancer using 67411-1-Ig

IHC staining of human liver cancer using 67411-1-Ig

IHC staining of human liver cancer using 67411-1-Ig

IF Staining of HepG2 using 67411-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.

IL-10 contributes to gemcitabine resistance in extranodal NK/T-cell lymphoma cells via ABCC4.

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma

Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I Interferon Responses

Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses