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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TYMS Monoclonal antibody (66725-1-Ig)

    WB analysis using 66725-1-Ig (same clone as 66725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66725-1-Ig (TYMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TYMS Monoclonal antibody (66725-1-Ig)

    WB analysis of HEK-293 using 66725-1-Ig (same clone as 66725-1-PBS)

    WB result of TYMS antibody (66725-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TYMS transfected HEK-293 cells. This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TYMS Monoclonal antibody (66725-1-Ig)

    IF Staining of HeLa using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TYMS antibody (66725-1-Ig, Clone: 1F5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using TYMS Monoclonal antibody (66725-1-Ig)

    FC experiment of HepG2 using 66725-1-Ig (same clone as 66725-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TYMS Monoclonal antibody (66725-1-Ig, Clone:1F5B7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TYMS Monoclonal antibody, PBS Only

TYMS Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66725-1-PBS
Clone No.1F5B7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Thymidylate synthase, OK/SW-cl.29, HsT422, EC:2.1.1.45, DKCD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66725-1-PBS targets TYMS in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TYMS fusion protein Ag7027 相同性解析による交差性が予測される生物種
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002567
Gene Symbol TYMS
Gene ID (NCBI) 7298
RRIDAB_2882076
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04818
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.