• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

TYMS Monoclonal antibody, PBS Only

TYMS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F5B7

Cat no : 66725-1-PBS

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using TYMS Monoclonal antibody (66725-1-Ig)

    WB analysis of HEK-293 using 66725-1-Ig (same clone as 66725-1-PBS)

    WB result of TYMS antibody (66725-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TYMS transfected HEK-293 cells. This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TYMS Monoclonal antibody (66725-1-Ig)

    WB analysis using 66725-1-Ig (same clone as 66725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66725-1-Ig (TYMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human pancreas cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:400 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYMS Monoclonal antibody (66725-1-Ig)

    IHC staining of human colon cancer using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (TYMS antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TYMS Monoclonal antibody (66725-1-Ig)

    IF Staining of HeLa using 66725-1-Ig (same clone as 66725-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TYMS antibody (66725-1-Ig, Clone: 1F5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66725-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66725-1-PBS targets TYMS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TYMS fusion protein Ag7027 相同性解析による交差性が予測される生物種
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002567
Gene symbol TYMS
Gene ID (NCBI) 7298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.