Talin-1 Recombinant antibody
Talin-1 Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
7D11
Cat no : 82856-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, C6 cells |
Positive IF/ICC detected in | A549 cells, HUVEC cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82856-4-RR targets Talin-1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種 |
Full Name | talin 1 |
Calculated molecular weight | 2541 aa, 270 kDa |
Observed molecular weight | 270 kDa |
GenBank accession number | BC042923 |
Gene symbol | Talin-1 |
Gene ID (NCBI) | 7094 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
Protocols
Product Specific Protocols | |
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WB protocol for Talin-1 antibody 82856-4-RR | Download protocol |
IF protocol for Talin-1 antibody 82856-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |