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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Talin-1 Recombinant antibody

Talin-1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

7D11

Cat no : 82856-4-RR

Synonyms

TLN, talin1, talin 1, KIAA1027, ILWEQ

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Talin-1 Recombinant antibody (82856-4-RR)

    WB analysis using 82856-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82856-4-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using Talin-1 Recombinant antibody (82856-4-RR)

    IHC staining of human colon using 82856-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Talin-1 Recombinant antibody (82856-4-RR)

    IHC staining of human colon using 82856-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Talin-1 Recombinant antibody (82856-4-RR)

    IF Staining of HUVEC using 82856-4-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-4-RR)

    IF Staining of A549 using 82856-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green).

  • Affinity and Kinetic Characterization of 82856-4-RR

    Affinity and Kinetic Characterization of 82856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82856-4-RR against Human TLN1 were performed. The affinity constant is 0.215 nM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82856-4-RR targets Talin-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight270 kDa
GenBank accession numberBC042923
Gene symbol Talin-1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
WB protocol for Talin-1 antibody 82856-4-RRDownload protocol
IHC protocol for Talin-1 antibody 82856-4-RRDownload protocol
IF protocol for Talin-1 antibody 82856-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols