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  • Featured Product
  • KD/KO Validated

Thioredoxin Monoclonal antibody, PBS Only

Thioredoxin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3C1

Cat no : 66475-1-PBS

Synonyms

ADF, ATL derived factor, SASP, thioredoxin, TRDX, TRX, TRX1, TXN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Thioredoxin Monoclonal antibody (66475-1-Ig)

    WB analysis using 66475-1-Ig (same clone as 66475-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66475-1-Ig (TXN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Thioredoxin Monoclonal antibody (66475-1-Ig)

    WB analysis using 66475-1-Ig (same clone as 66475-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66475-1-Ig (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Thioredoxin Monoclonal antibody (66475-1-Ig)

    WB analysis of HeLa using 66475-1-Ig (same clone as 66475-1-PBS)

    WB result of Thioredoxin antibody (66475-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells. This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Thioredoxin Monoclonal antibody (66475-1-Ig)

    IHC staining of human ovary tumor using 66475-1-Ig (same clone as 66475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Thioredoxin Monoclonal antibody (66475-1-Ig)

    IHC staining of human ovary tumor using 66475-1-Ig (same clone as 66475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Thioredoxin Monoclonal antibody (66475-1-Ig)

    IHC staining of human liver cancer using 66475-1-Ig (same clone as 66475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Thioredoxin Monoclonal antibody (66475-1-Ig)

    IF Staining of HepG2 using 66475-1-Ig (same clone as 66475-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Thioredoxin antibody (66475-1-Ig, Clone: 1F3C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66475-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66475-1-PBS targets Thioredoxin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thioredoxin fusion protein Ag6355 相同性解析による交差性が予測される生物種
Full Name thioredoxin
Calculated molecular weight 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC003377
Gene symbol TXN
Gene ID (NCBI) 7295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.