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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Thioredoxin Recombinant antibody

Thioredoxin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7G10

Cat no : 82765-1-RR

Synonyms

TXN, THIO, Surface-associated sulphydryl protein, Hom s Trx, ATL-derived factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Thioredoxin Recombinant antibody (82765-1-RR)

    WB analysis using 82765-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Thioredoxin Recombinant antibody (82765-1-RR)

    WB analysis using 82765-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Thioredoxin Recombinant antibody (82765-1-RR)

    IHC staining of human stomach using 82765-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82765-1-RR (Thioredoxin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82765-1-RR

    Affinity and Kinetic Characterization of 82765-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82765-1-RR against Human Thioredoxin were performed. The affinity constant is 0.437 nM.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82765-1-RR targets Thioredoxin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Thioredoxin fusion protein Ag6989 相同性解析による交差性が予測される生物種
Full Name thioredoxin
Calculated molecular weight 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC003377
Gene symbol TXN
Gene ID (NCBI) 7295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Protocols

Product Specific Protocols
WB protocol for Thioredoxin antibody 82765-1-RRDownload protocol
IHC protocol for Thioredoxin antibody 82765-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols