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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse placenta tissue using CD141/Thrombomodulin Recombinant antibody (84412-3-RR)

    WB analysis of mouse placenta using 84412-3-RR (same clone as 84412-3-PBS)

    mouse placenta tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using CD141/Thrombomodulin Recombinant antibody (84412-3-RR)

    WB analysis of mouse lung using 84412-3-RR (same clone as 84412-3-PBS)

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 84412-3-RR (CD141/Thrombomodulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01300-2

    Sandwich ELISA standard curve of MP01300-2

    Sandwich ELISA standard curve of MP01300-2, Mouse CD141/Thrombomodulin Recombinant Matched Antibody Pair - PBS only. 84412-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1139. 84412-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP01300-2

    Sample test of MP01300-2

    Mouse lung, cut into 1-2 mm pieces, wa scultured for 4 daysin 25-30 mL of RPMI supplemented with 10% fetal bovine serum. The cell culture supernate was removed, assayed for CD141/Thrombomodulin, and measured 34.22 ng/mL.

  • Sample test of MP01300-2

    Sample test of MP01300-2

    Serum of six mice was measured. The mouse CD141/Thrombomodulin concentration of detected samples was determined to be 28.01 ng/mL with a range of 21.90 - 32.07 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using CD141/Thrombomodulin Recombinant antibody (84412-3-RR)

    IF Staining of bEnd.3 using 84412-3-RR (same clone as 84412-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD141/Thrombomodulin antibody (84412-3-RR, Clone: 241730D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84412-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01300-1

    Cytometric bead array standard curve of MP01300-1

    Cytometric bead array standard curve of MP01300-1, MOUSE CD141/Thrombomodulin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84412-3-PBS. Detection antibody: 84412-2-PBS. Standard: Eg1139. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01300-2

    Cytometric bead array standard curve of MP01300-2

    Cytometric bead array standard curve of MP01300-2, MOUSE CD141/Thrombomodulin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84412-3-PBS. Detection antibody: 84412-1-PBS. Standard: Eg1139. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84412-3-RR

    Affinity and Kinetic Characterization of 84412-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84412-3-RR against Mouse CD141/Thrombomodulin were performed. The affinity constant is 3.11 nM.

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Mouse CD141/Thrombomodulin Recombinant antibody, PBS Only (Capture)

CD141/Thrombomodulin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84412-3-PBS
Clone No.241730D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TM, Thrombomodulin, Thbd, Fetomodulin, CD141

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84412-3-PBS targets CD141/Thrombomodulin as part of a matched antibody pair:

MP01300-1: 84412-3-PBS capture and 84412-2-PBS detection (validated in Cytometric bead array)

MP01300-2: 84412-3-PBS capture and 84412-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name thrombomodulin
Calculated molecular weight62kDa
Observed molecular weight105 kDa
GenBank accession numberNM_009378.3
Gene Symbol Thbd
Gene ID (NCBI) 21824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15306
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).