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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Thrombospondin 1 Monoclonal antibody (67241-1-Ig)

    WB analysis using 67241-1-Ig (same clone as 67241-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67241-1-Ig (THBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67241-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using Thrombospondin 1 Monoclonal antibody (67241-1-Ig)

    WB analysis of rat spleen using 67241-1-Ig (same clone as 67241-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67241-1-Ig (THBS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67241-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Thrombospondin 1 Monoclonal antibody (67241-1-Ig)

    WB analysis of NIH/3T3 using 67241-1-Ig (same clone as 67241-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67241-1-Ig (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67241-1-PBS in a different storage buffer formulation.

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Thrombospondin 1 Monoclonal antibody, PBS Only

Thrombospondin 1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67241-1-PBS
Clone No.2A3E1

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, Indirect ELISA

Synonyms

THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67241-1-PBS targets Thrombospondin 1 in WB, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Thrombospondin 1 fusion protein Ag29129 相同性解析による交差性が予測される生物種
Full Name thrombospondin 1
Calculated molecular weight 129 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_003246
Gene Symbol Thrombospondin 1
Gene ID (NCBI) 7057
RRIDAB_2882520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07996
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.