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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tim23 Monoclonal antibody, PBS Only

Tim23 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H10F5

Cat no : 67535-1-PBS

Synonyms

PRO1197, TIM23, TIMM23

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tim23 Monoclonal antibody (67535-1-Ig)

    WB analysis using 67535-1-Ig (same clone as 67535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Tim23 Monoclonal antibody (67535-1-Ig)

    IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Tim23 Monoclonal antibody (67535-1-Ig)

    IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Tim23 Monoclonal antibody (67535-1-Ig)

    IF Staining of HepG2 using 67535-1-Ig (same clone as 67535-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67535-1-PBS targets Tim23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Tim23 fusion protein Ag27069 相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC062707
Gene symbol Tim23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.