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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Timp-3 Recombinant antibody (83627-1-RR)

    WB analysis of human placenta using 83627-1-RR (same clone as 83627-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83627-1-RR (Timp-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MDA-MB-231 cells using Timp-3 Recombinant antibody (83627-1-RR)

    FC experiment of MDA-MB-231 using 83627-1-RR (same clone as 83627-1-PBS)

    1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Timp-3 Recombinant antibody (83627-1-RR)

    FC experiment of HeLa using 83627-1-RR (same clone as 83627-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83627-1-RR

    Affinity and Kinetic Characterization of 83627-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83627-1-RR against Human Timp-3 were performed. The affinity constant is 2.63 nM.

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Timp-3 Recombinant antibody, PBS Only

Timp-3 Uni-rAbTM Recombinant Antibody for ELISA, WB
Cat No. 83627-1-PBS
Clone No.240374D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA, WB

Synonyms

TIMP3, Protein MIG 5, Metalloproteinase inhibitor 3, K222TA2, K222

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83627-1-PBS targets Timp-3 in ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Timp-3 fusion protein Ag34657 相同性解析による交差性が予測される生物種
Full Name TIMP metallopeptidase inhibitor 3
Calculated molecular weight 24 kDa
GenBank accession numberBC014277
Gene Symbol TIMP3
Gene ID (NCBI) 7078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35625
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.