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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tissue Factor Polyclonal antibody (31453-1-AP)

    WB analysis using 31453-1-AP (same clone as 31453-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31453-1-AP (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31453-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Tissue Factor Polyclonal antibody, PBS Only

Tissue Factor Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31453-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31453-1-PBS targets Tissue Factor in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name coagulation factor III (thromboplastin, tissue factor)
Calculated molecular weight33 kDa
Observed molecular weight45-53 kDa
GenBank accession numberNM_001993.5
Gene Symbol F3
Gene ID (NCBI) 2152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP13726-1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).