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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Tissue Factor/CD142 Rabbit Recombinant (98104-1-RR)

    FC experiment of human PBMCs using 98104-1-RR

    1x10^6 untreated or LPS-treated human PBMCs were surface stained with 0.25 ug Anti-Human Coagulation Factor III/Tissue Factor Rabbit Recombinant Antibody (98104-1-RR, Clone:240784E2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98104-1-RR

    Affinity and Kinetic Characterization of 98104-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98104-1-RR against Human Tissue Factor/CD142 were performed. The affinity constant is 0.834 nM.

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Anti-Human Tissue Factor/CD142 Rabbit Recombinant Antibody

Tissue Factor/CD142 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98104-1-RR
Clone No.240784E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

F3, Coagulation factor III, CD142, Tissue factor, TFA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inLPS treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98104-1-RR targets Tissue Factor/CD142 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name coagulation factor III (thromboplastin, tissue factor)
Calculated molecular weight33 kDa
GenBank accession numberNM_001993.5
Gene Symbol F3
Gene ID (NCBI) 2152
RRIDAB_3672251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13726-1
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named CD142, coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

Protocols

Product Specific Protocols
FC protocol for Tissue Factor/CD142 antibody 98104-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols