TLR4 Polyclonal antibody
TLR4 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 30400-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | J774A.1 cells, THP-1 cells, NR8383 cells, RAW 264.7 cells, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
30400-1-AP targets TLR4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TLR4 fusion protein Ag32998 相同性解析による交差性が予測される生物種 |
| Full Name | toll-like receptor 4 |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 96 kDa |
| GenBank accession number | NM_021297 |
| Gene symbol | Tlr4 |
| Gene ID (NCBI) | 21898 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TLR4, also known as CD284, belongs to the Toll-like receptor family. It is a type I transmembrane protein consisting of an extracellular leucine-rich repeat region, a helix transmembrane domain, and an intracellular Toll-IL-1 receptor domain. TLR4 functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. It interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TLR4 antibody 30400-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Heliyon Asprosin promotes vascular inflammation via TLR4-NFκB-mediated NLRP3 inflammasome activation in hypertension | ||
Int J Immunopathol Pharmacol Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells
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