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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tom22 Monoclonal antibody (66562-1-Ig)

    WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Tom22 Monoclonal antibody (66562-1-Ig)

    WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Tom22 Monoclonal antibody (66562-1-Ig)

    IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Tom22 Monoclonal antibody (66562-1-Ig)

    IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Tom22 Monoclonal antibody (66562-1-Ig)

    IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Tom22 Monoclonal antibody (66562-1-Ig)

    IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Tom22 Monoclonal antibody (66562-1-Ig)

    IHC staining of human colon cancer using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Tom22 Monoclonal antibody (66562-1-Ig)

    IHC staining of human colon cancer using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Tom22 Monoclonal antibody (66562-1-Ig)

    IF Staining of HepG2 using 66562-1-Ig (same clone as 66562-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tom22 antibody (66562-1-Ig, Clone: 1C2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using Tom22 Monoclonal antibody (66562-1-Ig)

    FC experiment of HEK-293 using 66562-1-Ig (same clone as 66562-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Tom22 (66562-1-Ig, Clone:1C2B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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Tom22 Monoclonal antibody, PBS Only

Tom22 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66562-1-PBS
Clone No.1C2B11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TOMM22, MSTP065, MST065, hTom22, 1C9 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66562-1-PBS targets Tom22 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Tom22 fusion protein Ag1797 相同性解析による交差性が予測される生物種
Full Name translocase of outer mitochondrial membrane 22 homolog (yeast)
Calculated molecular weight 15 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009363
Gene Symbol TOMM22/Tom22
Gene ID (NCBI) 56993
RRIDAB_2881923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS69
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).