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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Transferrin Recombinant antibody, PBS Only

Transferrin Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5O7

Cat no : 81676-1-PBS

Synonyms

Beta 1 metal binding globulin, DKFZp781D0156, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Transferrin Recombinant antibody (81676-1-RR)

    WB analysis using 81676-1-RR (same clone as 81676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81676-1-RR (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81676-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Transferrin Recombinant antibody (81676-1-RR)

    WB analysis using 81676-1-RR (same clone as 81676-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81676-1-RR (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Transferrin Recombinant antibody (81676-1-RR)

    IHC staining of human hepatocirrhosis using 81676-1-RR (same clone as 81676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 81676-1-RR (Transferrin antibody) at dilution of 1:950 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81676-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Transferrin Recombinant antibody (81676-1-RR)

    IHC staining of human hepatocirrhosis using 81676-1-RR (same clone as 81676-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 81676-1-RR (Transferrin antibody) at dilution of 1:950 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81676-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81676-1-PBS targets Transferrin in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Transferrin fusion protein Ag11504 相同性解析による交差性が予測される生物種
Full Name transferrin
Calculated molecular weight 698 aa, 77 kDa
Observed molecular weight77 kDa
GenBank accession numberBC059367
Gene symbol TF
Gene ID (NCBI) 7018
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.