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  • Featured Product
  • KD/KO Validated

Transferrin/TF Polyclonal antibody, PBS Only

Transferrin/TF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17435-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Transferrin/TF Polyclonal antibody (17435-1-AP)

    WB analysis using 17435-1-AP (same clone as 17435-1-PBS)

    human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    WB analysis of rat kidney using 17435-1-AP (same clone as 17435-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    WB analysis of mouse kidney using 17435-1-AP (same clone as 17435-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    WB analysis of mouse kidney using 17435-1-AP (same clone as 17435-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    WB analysis of mouse spleen using 17435-1-AP (same clone as 17435-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    WB analysis of rat kidney using 17435-1-AP (same clone as 17435-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human hepatocirrhosis using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human hepatocirrhosis using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human colon using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17435-1-AP (Transferrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human testis using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human placenta using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human placenta using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IHC staining of human testis using 17435-1-AP (same clone as 17435-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Transferrin/TF Polyclonal antibody (17435-1-AP)

    IF Staining of HepG2 using 17435-1-AP (same clone as 17435-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17435-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17435-1-PBS targets Transferrin/TF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Transferrin/TF fusion protein Ag11504 相同性解析による交差性が予測される生物種
Full Name transferrin
Calculated molecular weight 698 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC059367
Gene symbol TF
Gene ID (NCBI) 7018
RRIDAB_2035023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273