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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    WB analysis of K-562 using 60044-1-Ig (same clone as 60044-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    WB analysis of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    WB analysis of MCF-7 using 60044-1-Ig (same clone as 60044-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    WB analysis of A549 using 60044-1-Ig (same clone as 60044-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IP experiment of HEK-293 using 60044-1-Ig (same clone as 60044-1-PBS)

    IP result of anti-Transgelin 2 (IP:60044-1-Ig, 4ug; Detection:60044-1-Ig 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human urothelial carcinoma using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of mouse colon using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of mouse colon using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 60044-1-Ig (Transgelin 2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human colon using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human colon using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IHC staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60044-1-Ig (Transgelin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IF Staining of human colon cancer using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Transgelin 2 Monoclonal antibody (60044-1-Ig)

    IF Staining of HepG2 using 60044-1-Ig (same clone as 60044-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transgelin 2 antibody (60044-1-Ig, Clone: 2E5G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60044-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Transgelin 2 Monoclonal antibody, PBS Only

Transgelin 2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 60044-1-PBS
Clone No.2E5G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e, CDABP0035

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60044-1-PBS targets Transgelin 2 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Transgelin 2 fusion protein Ag0337 相同性解析による交差性が予測される生物種
Full Name transgelin 2
Calculated molecular weight 199 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002616
Gene Symbol Transgelin-2/TAGLN2
Gene ID (NCBI) 8407
RRIDAB_2271433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP37802
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.