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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Transthyretin Polyclonal antibody (31130-1-AP)

    WB analysis using 31130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31130-1-AP (Transthyretin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Transthyretin Polyclonal antibody

Transthyretin Polyclonal Antibody for WB, ELISA
Cat No. 31130-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman heart tissue, human plasma tissue, mouse brain tissue, mouse liver tissue, rat plasma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31130-1-AP targets Transthyretin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name transthyretin
Calculated molecular weight16 kDa
Observed molecular weight15 kDa
GenBank accession numberNM_000371.4
Gene Symbol TTR
Gene ID (NCBI) 7276
RRIDAB_3669866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. However Western blot analysis using specific rabbit anti-mouse TTR reveals that TTR is absent from the cortex, hippocampus, and cerebellum and present in the choroid plexus. (PMID: 22103483, 16698124)

Protocols

Product Specific Protocols
WB protocol for Transthyretin antibody 31130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols