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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TrkB Polyclonal antibody

TrkB Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29961-1-AP

Synonyms

GP145 TrkB, NTRK2, Trk B, TRKB, TrkB tyrosine kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TrkB Polyclonal antibody (29961-1-AP)

    WB analysis using 29961-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (NTRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TrkB Polyclonal antibody (29961-1-AP)

    WB analysis using 29961-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29961-1-AP (TrkB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TrkB Polyclonal antibody (29961-1-AP)

    IHC staining of mouse cerebellum using 29961-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TrkB Polyclonal antibody (29961-1-AP)

    IHC staining of mouse cerebellum using 29961-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (TrkB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TrkB Polyclonal antibody (29961-1-AP)

    IHC staining of mouse brain using 29961-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TrkB Polyclonal antibody (29961-1-AP)

    IHC staining of mouse brain using 29961-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TrkB Polyclonal antibody (29961-1-AP)

    IHC staining of mouse cerebellum using 29961-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TrkB Polyclonal antibody (29961-1-AP)

    IHC staining of mouse cerebellum using 29961-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29961-1-AP (NTRK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TrkB Polyclonal antibody (29961-1-AP)

    IF Staining of mouse brain using 29961-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TrkB antibody (29961-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29961-1-AP targets TrkB in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TrkB fusion protein Ag32196 相同性解析による交差性が予測される生物種
Full Name neurotrophic tyrosine kinase, receptor, type 2
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 90-95 kDa, 140-145 kDa
GenBank accession numberBC031835
Gene symbol TrkB
Gene ID (NCBI) 4915
RRIDAB_2918354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 238-430 aa of human TrkB recognizes full length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Protocols

Product Specific Protocols
WB protocol for TrkB antibody 29961-1-APDownload protocol
IHC protocol for TrkB antibody 29961-1-APDownload protocol
IF protocol for TrkB antibody 29961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols