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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TrkB Recombinant antibody

TrkB Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241473G5

Cat no : 80878-6-RR

Synonyms

NTRK2, NTRK 2, GP145-TrkB, GP145 TrkB, BDNF/NT-3 growth factors receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TrkB Recombinant antibody (80878-6-RR)

    WB analysis using 80878-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80878-6-RR (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse cerebellum tissue using TrkB Recombinant antibody (80878-6-RR)

    IP experiment of mouse cerebellum using 80878-6-RR

    IP result of anti-TrkB (IP:80878-6-RR, 4ug; Detection:80878-6-RR 1:4000) with mouse cerebellum tissue lysate 2000 ug.

  • Affinity and Kinetic Characterization of 80878-6-RR

    Affinity and Kinetic Characterization of 80878-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80878-6-RR against Human TrkB were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inrat brain tissue, mouse cerebellum tissue
Positive IP detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 80878-6-RR is TrkB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TrkB fusion protein Ag3790 相同性解析による交差性が予測される生物種
Full Name neurotrophic tyrosine kinase, receptor, type 2
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC031835
Gene symbol TrkB
Gene ID (NCBI) 4915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Protocols

Product Specific Protocols
WB protocol for TrkB antibody 80878-6-RRDownload protocol
IP protocol for TrkB antibody 80878-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols