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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tyrosinase Polyclonal antibody

Tyrosinase Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31291-1-AP

Synonyms

TYR, OCAIA, OCA1A, Monophenol monooxygenase, LB24-AB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tyrosinase Polyclonal antibody (31291-1-AP)

    WB analysis using 31291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31291-1-AP (Tyrosinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Tyrosinase Polyclonal antibody (31291-1-AP)

    IHC staining of human skin cancer using 31291-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 31291-1-AP (Tyrosinase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA375 cells, mouse liver tissue, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31291-1-AP targets Tyrosinase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tyrosinase fusion protein Ag34940 相同性解析による交差性が予測される生物種
Full Name tyrosinase (oculocutaneous albinism IA)
Calculated molecular weight 529 aa, 60 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC027179
Gene symbol TYR
Gene ID (NCBI) 7299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941)

Protocols

Product Specific Protocols
WB protocol for Tyrosinase antibody 31291-1-APDownload protocol
IHC protocol for Tyrosinase antibody 31291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols